Viral RNA ExtractMe Viral RNA kit suitable for COVID-19
The EXTRACTME VIRAL RNA KIT is designed for the rapid and efficient purification of high quality viral RNA from swabs. The kit is specifically designed to isolate viral nucleic acid from a variety of RNA viruses including SARS-CoV-2 (the virus that causes COVID-19). The isolation protocol and buffer formulation were optimized for high isolation efficiency and RNA purity.
The EXTRACTME VIRAL RNA KIT utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. During the first isolation step, the material is lysed under highly denaturing conditions to inactivate nucleases and to ensure isolation of intact viral RNA. A homogenate is lysed with guanidine thiocyanate and detergents. RNases are inactivated by guanidine thiocyanate. RNA bounds to a Purification Column membrane by addition of alcohol. A three-step washing stage effectively removes impurities and enzyme inhibitors. Purified RNA is eluted with the use of low ionic strength buffer and may be used directly in all downstream applications, such as RT-PCR, RT-qPCR, cDNA synthesis.
swab in viral transport medium (e.g., nasopharyngeal swabs, mouth and throat swabs)
dependent on the sample material amount and type
~ 120 μg RNA
A260/A280 ratio = 1.9 – 2.1