BLIRT ExtractMe Total RNA 96-well Kit

High-throughput total RNA extraction from tissue and cell lines in 96-well format.

ExtractMe Total RNA 96-well Kit
Product Part Number Pack Size
ExtractMe Total RNA 96-well Kit  SWA- EM14-192 2x 96 preps
SWA-EM14-960 10x 96 preps

 

The EXTRACTME TOTAL RNA 96-WELL kit is designed for high-throughput and efficient purification of high quality RNA from up to 10 mg of tissue (fresh or frozen) and 104 - 107 cultured cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and purity of RNA. The product is intended for research use only.

SAMPLE MATERIAL

- fresh or frozen tissue (stored at -80°C): 1-10 mg
- tissue preserved in RNase inactivating buffers: 1-10 mg
- cell culture: 104 - 107 cells

YIELD

up to 35 µg RNA per well

BINDING CAPACITY

70 μg RNA

TIME REQUIRED

45 min/plate (lysis time not included)

RNA PURITY

A260/A280 ratio = 1.9 – 2.1

PLATE FORMAT

standard SBS footprint

EQUIPMENT COMPATIBILITY

- Centrifuge with rotor for plates (≥3k x g);

- Automatic Liquid Handling Systems (eg. Biomek® FX, TECAN Freedom Evo, TECAN Genesis, Eppendorf epMotion)

Principle

The EXTRACTME TOTAL RNA 96-WELL kit utilizes 96-minicolumn plates with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 5 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed with guanidine thiocyanate and detergents. Any RNases are inactivated by guanidine thiocyanate and β-mercaptoethanol. The RNA is bound to the Purification Minicolumn plate
membrane by addition of ethanol. The three-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water (pH 7.0-9.0) and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth.

Quality control

The quality of each production batch (LOT) of the EXTRACTME TOTAL RNA 96-WELL kit is tested using standard QC procedures. The purified RNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. In addition, the functional quality is tested by reverse transcription and qPCR.

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