BLIRT TaqNovaGC Polymerase

Taq polymerase ideal for amplification on GC-rich templates

TaqNovaGC Polymerase
Name Part Number Pack Size
TaqNovaGC Polymerase RP73-020 200 U (5 U/µl)
  RP73-100 100 U (5 U/µl)

 

TaqNovaGC DNA Polymerase is an ideal tool for the amplification of GC-rich templates. A recombinant and  thermostable enzyme isolated from Thermus aquaticus, it is recommended for a wide range of applications requiring DNA synthesis at extremely high temperatures. The TaqNovaGC DNA polymerase is a universal DNA polymerase which works rapidly and effectively in various PCR conditions. The enzyme catalyses DNA synthesis in a 5’-3’ direction, shows no 3’-5’ exonuclease activity, but has a 5’-3’ exonuclease activity.

 

The application of appropriate PCR buffer conditions and 5x GC-Additive as the PCR enhancer enables the amplification of specific DNA regions with a high GC-content. This PCR additive changes DNA behavior upon heating and can be used with primer-template pairs with a GC-rich content which do not work well under standard PCR conditions. The 5x GC-Additive reduces the number of secondary structures and enables the specific hybridization of primers.

 

Features and advantages

- Recombinant enzyme of high purity

- Half-life of the enzyme is 45 minutes at 95°C

- Amplifies fragments of up to 5 kb

- Leaves ´A´ overhangs

- High specificity

- Ideal for problematic templates, which fail with standard Taq DNA polymerases

 

Applications

- Efficient amplification of short and medium size DNA sequences

- GC-rich templates

- Ideal for problematic templates, which fail with standard Taq DNA polymerases

Storage buffer

20 mM Tris-HCl (pH 8.0 w 25°C), 200 mM KCl, 0.1 mM EDTA, 2 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol

Quality control

Free of nonspecific nucleases (DNases and RNases). Extensively tested for nicking activity and in PCR applications focusing on templates rich in GC.

Storage conditions

Store all components at -20°C.

Shipping conditions

Shipping on dry ice

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