BLIRT LongNova DNA Polymerase
Blend of Taq & Pwo polymerases; long range (up to 20 kb)
|Name||Part Number||Pack Size|
|LongNova DNA Polymerase||RP281||200 U|
LongNova DNA Polymerase is a mixture of thermostable Pwo and Taq polymerases. The thermostable LongNova DNA Polymerase catalyses DNA synthesis in a 5’-3’ direction and shows a 3’-5’ exonuclease activity. It is characterized by high processivity and proofreading properties. It is ideal for long range PCR (up to 20 kb) and GC-rich template amplification applications.
Features and advantages
- Wide range of product sizes – from 2 to 20 kb
- High proofreading properties (3’-5’ exonuclease activity)
- Long PCR products
- Molecular cloning
- Site-directed mutagenesis and other methods which require high fidelity
20 mM Tris-HCl (pH 8.0 w 25°C), 200 mM KCl, 0.1 mM EDTA, 2 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol
Free of unspecific nucleases and DNA contamination. The amplification and proofreading activities of the polymerase are tested in appropriate assays. Extensively tested in various long range PCR reactions.
One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.
Store all components at -20°C.
Shipping on dry ice