BLIRT LongNova DNA Polymerase

Blend of Taq & Pwo polymerases; long range (up to 20 kb)

LongNova DNA Polymerase
Name Part Number Pack Size
LongNova DNA Polymerase RP281 200 U 
  RP282 1000 U

 

LongNova DNA Polymerase is a mixture of thermostable Pwo and Taq polymerases. The thermostable LongNova DNA Polymerase catalyses DNA synthesis in a 5’-3’ direction and shows a 3’-5’ exonuclease activity. It is characterized by high processivity and proofreading properties. It is ideal for long range PCR (up to 20 kb) and GC-rich template amplification applications.  

 

Features and advantages

- Wide range of product sizes – from 2 to 20 kb

- High proofreading properties (3’-5’ exonuclease activity)

 

Applications

- Long PCR products

- Molecular cloning

- Site-directed mutagenesis and other methods which require high fidelity

Storage buffer

20 mM Tris-HCl (pH 8.0 w 25°C), 200 mM KCl, 0.1 mM EDTA, 2 mM DTT, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20, 50% (v/v) glycerol

Quality control

Free of unspecific nucleases and DNA contamination. The amplification and proofreading activities of the polymerase are tested in appropriate assays. Extensively tested in various long range PCR reactions.

Unit definition

One unit is defined as an amount of enzyme required to incorporate 10 nmol of dNTPs to an insoluble DNA fraction in 30 minutes at 75°C in a 50 μl reaction.

Storage conditions

Store all components at -20°C.

Shipping conditions

Shipping on dry ice

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