BLIRT Lysostaphin

Zinc metalloproteinase extracted from Staphylococcus simulans biovar staphylolyticus that degrades the cell wall of almost all known staphylococcal species

Lysostaphin
Name Part Number Pack Size
Lysostaphin RP12 400 U
  RP125 2000 U

 

Lysostaphin is a zinc metalloproteinase extracted from Staphylococcus simulans biovar staphylolyticus that degrades the cell wall of almost all known staphylococcal species [1,2]. The target of the lysostaphin activity is the pentaglycine interpeptide bridges of the unique staphylococcal peptidoglycan, where the enzyme cleaves the Gly-Gly bond and lyses cells in all metabolic states (growing, resting, or heat killed). The specificity of the pentaglycine peptide is very high and other gram–positive and gram–negative bacteria are not susceptible to this enzyme. The unique biological activity of lysostaphin presents numerous possibilities for applications of this enzyme in the medical, veterinary, food industry and research fields [3]. Lysostaphin is frequently used as a staphylolytic agent for the liberation of intracellular enzymes, nucleic acids, and cell membrane and surface components. Lysostaphin was tested as a chemotherapeutic agent. Lysostaphin application was also shown to be effective in reducing the nasal carriage of S. aureus in humans. There are also examples for application of this enzyme in food protection.

Properties

Activity: 400 u/ml

Concentration: 1 mg/ml

Electrophoretic purity: >95%

Protocol: DNA isolation from Staphylococcus species is supplied

Applications

- frequently used as a staphylolytic agent for the liberation of intracellular enzymes, nucleic acids, and cell membrane and surface components 

- preparation of spheroplasts

References

1. Schindler CA, Schuhardt VT, Lysostaphin: a new bacteriolytic agent for the Staphylococcus., Proc. Natl. Acad. Sci. USA, 1964, 51, 414-21

2. Schindler CA, Schuhardt VT, Purification and properties of lysostaphin: a lytic agent for Staphylococcus aureus., Biochem. Acta., 1965, 97, 242-50.

3. Szweda P, Kotłowski R, Kur J, New effective sources of the Staphylococcus simulans lysostaphin., J. Biotechnol., 2005, 117, 203-13.

Storage Buffer

10 mM Tris-HCl (pH 7.5), 0.5 M NaCl, 50% (v/v) glycerol

Storage Temperature

 -20°C

Shipping with Dry Ice. 

Unit Definition

One unit of the enzyme causes an increase in absorbance of 0.1 per 10 minutes at 37°C and pH 7.5 due to lysis S. aureus.

Guarantee

6 months from the purchase date


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