BLIRT Ideal ready-to-use DNA Ladder

DNA fragment lengths (bp):

9276, 5982, 3653, 2555, 1827, 1241, 900, 700

Ready-to-use - premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels

Ideal ready-to-use DNA Ladder
Ideal ready-to-use DNA Ladder
Ideal ready-to-use DNA Ladder
Ideal ready-to-use DNA Ladder Ideal ready-to-use DNA Ladder Ideal ready-to-use DNA Ladder
Name Part Number Pack Size
Ideal ready-to-use DNA Ladder MR95 100 lanes

 

Molecular weight standard specially designed for electrophoretic analysis of medium and large DNA fragments on agarose and non-denaturing polyacrylamide gels. Ideal for sizing of PCR products, digested plasmids and medium or large double-stranded DNA fragments. IDEAL DNA ladder contain purified blunt-ended PCR products and cohesive-ended restriction fragments. The ladder is premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels.The optimized 6x Green DNA Loading Dye contains two electrophoresis tracking dyes: orange G and xylene cyanol FF. Orange G migrates at approximately 50 bp on a standard 1% TAE or TBE agarose gels, whereas xylene cyanol FF migrates at approx. 4 kbp.

Contents

- IDEAL ready-to-use DNA Ladder (250 µl)

- 6x Green DNA Loading Dye (500 µl)

Features

- ideal, because in wide range clear

- ready-to-use - premixed with 6x Green DNA Loading Dye for direct loading onto agarose and non-denaturing polyacrylamide gels 

- allow precise sizing of medium and large DNA fragments

- produce sharp bands during gel electrophoresis

- can be visualized under UV light after ethidium bromide, SYBR Green or SYBR Gold staining

- loading dye solution is included

DNA fragment lengths (bp):

9276, 5982, 3653, 2555, 1827, 1241, 900, 700

Storage Buffer

10 mM Tris-HCl (pH 7.6), 0.005% xylene cyanol FF, 0.025% orange G, 2.5% Ficoll® 400 and 10 mM EDTA

6x Green DNA Loading Dye (supplied with the ladder)

20 mM Tris-HCl (pH 7.6), 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA

Loading

- we recommend loading 5 µl of ready-to-use DNA ladder per gel lane

- do not heat before loading

- mix 1 volume of the dye with 5 volumes of the DNA sample

Quality Control

- tested in appropriate gel electrophoresis

- the absence of nucleases is confirmed by appropriate tests

Storage

Store at room temperature or at 4°C. For longer periods, store at -20°C.

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