BLIRT ExtractMe DNA Bacteria Kit
Rapid and efficient purification of high quality bacterial DNA from broth and plate cultures as well as frozen cells; binding capacity 40ug DNA; 40-60 minutes (including incubation time)
|Product||Pack Size||Cat No.|
|ExtractMe DNA Bacteria kit||50 preps||SWA-EM02-050|
The EXTRACTME DNA BACTERIA kit is designed for the rapid and efficient purification of high quality bacterial DNA from broth and plate cultures as well as frozen cells. The isolation protocol and buffer formulations were optimised for high isolation efficiency and DNA purity.
The DNA purification procedure consists of four steps and utilises spin minicolumns with membranes which efficiently and selectively bind nucleic acids. In the first isolation step, the cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. To obtain an RNA-free DNA sample, the RNA is removed by RNase A. After the addition of chaotropic salts, the lysate is applied to the purification column membrane and the DNA is bound. The two-step washing stage effectively removes impurities and enzyme inhibitors. The purified DNA is eluted using a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use.
The quality of each production batch (LOT) of the EXTRACTME DNA BACTERIA kit is tested using standard QC procedures. The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. In addition, the functional quality is tested by qPCR and digestion with restriction enzymes.
Broth or plate bacterial culture, frozen cell pellet.
Up to 5 x 108 cells → 100%
109 ÷ 3 x 109 → 75-90% (when the modified protocol for isolation from large number of cells is followed)
109 ÷ 3 x 109 → ≤ 60% (when the standard isolation protocol is followed)
Approx. 40 μg DNA
40-60 minutes (including incubation time)
A260/A280 ratio = 1.7 – 1.9