BLIRT EasyPCR XY - human sex determination using PCR (+ DNA extraction)

Aim: to carry out a genomic DNA isolation from an oral swab and conduct PCR reactions with the isolated templates in order to determine human sex. Set with reagents for DNA extraction.

EasyPCR XY - human sex determination using PCR (+ DNA extraction)
EasyPCR XY - human sex determination using PCR (+ DNA extraction)
EasyPCR XY - human sex determination using PCR (+ DNA extraction) EasyPCR XY - human sex determination using PCR (+ DNA extraction)
Name Part Number Pack Size
EasyPCR XY - human sex determination using PCR (+ DNA extraction) DY10 1 exercise
  DY105 5 exercises

 

EasyPCR XY - educational kit for human sex determination by PCR reaction.

AIM: The purpose of the exercise is to carry out a genomic DNA isolation from buccal swabs and a PCR reaction with the isolated DNA templates in order to determine human sex using PCR. The procedure is based on the amplification of a human amelogenin gene fragment. This gene is shorter by 189bp on chromosome Y as compared with chromosome X, which allows for the determination of human sex by means of PCR. The expected PCR products are 977bp and 788bp long for males, or only 977bp for females.

The kit is designed to perform experiments during two or three laboratory classes (I - DNA extraction and PCR set-up - 2-2.5 hours; II - electrophoresis - 1.5-2 hours or I - DNA extraction - 1-1.5 hours; II - PCR set-up - 1.5 hours; III - electrophoresis - 1.5-2 hours). We suggest to perform laboratory experiments in six groups of two students.

EasyPCR XY kit components (1 exercise, DY10)

A) 6 sets of reagents for  DNA isolation from buccal swabs

(each set for 4 isolations)

B) 6 sets of PCR reagents:  

- 6 tubes with 10x reaction buffer (10 reactions per tube)

- 6 tubes with MgCl2 solution (10 reactions per tube)

- 6 tubes with ready-to-use dNTPs mixture (10 reactions per tube)

- 6 tubes with FORWARD primer (10 reactions per tube)

- 6 tubes with REVERSE primer (10 reactions per tube)

- 6 tubes with PCR - grade water

- 6 tubes with thermostable DNA polymerase (10 reactions per tube)

- 6 tubes with Positive controls (5 reactions per tube) with MALE and FEMALE genomic DNA

C) Moreover:

- 7 laboratory protocols (one with additional information for a teacher)

- Agarose (5 g)

- 50x TAE buffer for agarose gel electrophoresis (50 ml)

- Ready-to-use M100-1000 DNA Ladder for 10 electrophoretic lanes

- 6x GREEN DNA Gel Loading Buffer (0.5 ml)

EasyPCR XY kit components (5 exercises, DY105):

A) 6 sets of reagents for  DNA isolation from buccal swabs

(each set for 20 isolations)

B) 6 sets of PCR reagents:  

- 6 tubes with 10x reaction buffer (50 reactions per tube)

- 6 tubes with MgCl2 solution (50 reactions per tube)

- 6 tubes with ready-to-use dNTPs mixture (50 reactions per tube)

- 6 tubes with FORWARD primer (50 reactions per tube)

- 6 tubes with REVERSE primer (50 reactions per tube)

- 6 tubes with PCR - grade water

- 6 tubes with thermostable DNA polymerase (50 reactions per tube)

- 6 tubes with Positive controls (25 reactions per tube) with MALE and FEMALE genomic DNA

C) Moreover:

- 7 laboratory protocols (one with additional information for a teacher)

- Agarose (25 g)

- 50x TAE buffer for agarose gel electrophoresis (100 ml)

- Ready-to-use M100-1000 DNA Ladder for 50 electrophoretic lanes

- 6x GREEN DNA Gel Loading Buffer (1 ml)

Additional equipment and materials required (not included in the kit):

- Automatic pipettes with appropriate tips

- PCR tubes and 1.5 ml Eppendorf tubes

- Sterile swab sticks

- Microcentrifuge
- Thermoblock, incubator or thermostatic water bath

- UV transilluminator or lamp

- Thermal cycler

- Microwave oven or laboratory burner

- Agarose electrophoresis equipment (apparatus, power supply)

- Ethidium bromide solution or other stain for DNA visualization

- Nitrile gloves

Shipping conditions

Shipping on dry or blue ice.