BLIRT 10X TBE (Tris-borate-EDTA)

Name Part Number Pack Size
10X TBE (Tris-borate-EDTA) AG19 250 mL

 

TBE (Tris-borate-EDTA) is a buffer used for the electrophoresis of nucleic acids. Thanks to its higher buffering capacity and lower electric conductivity in comparison to a TAE buffer, the TBE buffer can be used for electrophoresis in both agarose and polyacrylamide gels. It is more suitable for the separation of smaller DNA fragments (< 500 bp) such as PCR products or restriction fragments. If an excision of a gel slice containing a DNA fragment, known as a DNA gel-out, is required, the use of the TAE buffer is recommended. The TBE buffer contains borate, which is a strong inhibitor against such enzymes as DNA ligase or DNA polymerase.

Applications

 - As a running buffer for the electrophoresis of nucleic acids in agarose and polyacrylamide gels

- Preparation of agarose and polyacrylamide gels

- High resolution electrophoresis of small DNA fragments

Formulation

0.89 M Tris (pH 8.3), 0.89 M boric acid, 20 mM EDTA

Quality control

The TBE buffer has been tested in electrophoretic runs utilising agarose and polyacrylamide gels. The absence of nucleases has been confirmed by the relevant QC procedures.

Storage conditions

Store at +4°C or room temperature.

Shipping conditions

Shipping at room temperature.

Usage

- Prior to use, the stock solution provided should be diluted to the working solution strength.

- In order to obtain a working solution (1x concentrated), dilute one part of the 10x TBE Buffer stock solution provided in 9 parts of distilled water; for example, add 450 ml of distilled water for every 50 ml of the stock solution.

- The preparation of a fresh working solution before each electrophoresis run is recommended.

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