BLIRT Gel Loading Dye - GREEN (6X)

Gel Loading Dye - GREEN (6X)
Name Part Number Pack Size
Gel Loading Dye - GREEN (6X) AG18 1 mL

 

6x Green DNA Gel Loading Dye is pre-mixed loading buffer with a tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. This solution contains EDTA that binds divalent metal ions, inhibits metal dependent enzymatic reactions and protects DNA from metal dependent nucleases, effectively inactivating enzymes and protecting DNA from nucleases. The loading dyes increase the density of the sample and they add colour to the sample, thereby simplifying the loading process. The optimized 6x Green DNA Loading Dye contains two electrophoresis tracking dyes: orange G and xylene cyanol FF. Orange G migrates at approximately 50 bp on a standard 1% TAE or TBE agarose gels, whereas xylene cyanol FF migrates at approx. 4 kbp.

Features

Orange G dye allows for the monitoring of DNA migration on agarose and polyacrylamide gels; it migrates with the smallest DNA fragments without masking them under UV light. Because xylene cyanol FF migrates much slower than orange G, it is useful as a tracking dye when running gels for an extended time - long after orange G has run off the bottom of the gel.

6x Green DNA Gel Loading Dye

20 mM Tris-HCl (pH 7.6), 0.03% xylene cyanol FF, 0.15% orange G, 15% Ficoll® 400 and 60 mM EDTA

Usage notes

Use 1 µl of 6x Green Gel Loading Dye per 5 µl of DNA sample. Mix well before loading onto a gel.

Quality Control

Tested for DNA samplepreparation prior to agarose gel electrophoresis.

Storage

Store at room temperature or at +4°C up to 12 months. For longer periods, store at -20°C.

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