BLIRT 10x TG (Tris-Glycine)

Name Part Number Pack Size
10x TG (Tris-Glycine) AC18 250 mL

 

TG (Tris-Glycine) is the buffer most commonly used for the non-denaturating polyacrylamide gel electrophoresis of proteins.

Applications

 Non-denaturating polyacrylamide gel electrophoresis of proteins

Formulation

0.25 M Tris (pH 8.3), 1.92 M glycine

Quality control

The TG buffer has been tested in electrophoretic runs utilizing agarose and polyacrylamide gels. The absence of nucleases and proteases has been confirmed by the relevant QC procedures.

Storage conditions

Store at +4°C or room temperature.

Shipping conditions

Shipping at room temperature

Usage

- Prior to use, the stock solution provided should be diluted to the working solution strength.

- In order to obtain a working solution (1x concentrated), dilute one part of the 10x TG buffer stock solution provided in 9 parts of distilled water; for example, add 450 ml of distilled water for every 50 ml of the stock solution.

- The preparation of a fresh working solution before each electrophoresis run is recommended.

- Non-denaturating polyacrylamide gel electrophoresis of proteins

Download Product Literature: